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lambda mouse 129-sv/j genomic dna library  (Agilent technologies)


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    Agilent technologies lambda mouse 129-sv/j genomic dna library
    Lambda Mouse 129 Sv/J Genomic Dna Library, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+129/pmc03990712-29-18-24
    Average 90 stars, based on 1 article reviews
    lambda mouse 129-sv/j genomic dna library - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: Altered neuronal lineages in the facial ganglia of Hoxa2 mutant mice.
    Article Snippet: .. The Hoxb1 locus was isolated from a 129 strain genomic lambda phage library (Stratagene) using a cDNA probe (Frohman et al., 1990). ..

    Article Title: Targeted disruption of the voltage-dependent calcium channel ? 2 /?-1-subunit
    Article Snippet: .. A 12-Kb genomic fragment containing exon 2 of the α 2 /δ-1 gene encoding residues I33-D59 [82 base pairs (bp)] was isolated and sequenced from a lambda mouse genomic DNA library (129 SVJ, Stratagene; La Jolla, CA). ..

    Labeling:

    Article Title: Gene expression profiling in the submandibular gland, stomach, and duodenum of CAVI-deficient mice.
    Article Snippet: Carbonic anhydrase VI (CAVI) is the only secreted isozyme of the a-carbonic anhydrase family, which catalyzes the reversible reaction CO2 þ H2O, HCO 3 þ Hþ.. It appears that CAVI protects teeth and gastrointestinal mucosa by neutralizing excess acidity.. However, the evidence for this physiological function is limited, and CAVI may have additional functions that have yet to be discovered.

    other:

    Article Title: Generation and Characterization of a Mouse Model Harboring the Exon-3 Deletion in the Cardiac Ryanodine Receptor
    Article Snippet: A genomic DNA phage clone containing part of the mouse cardiac ryanodine receptor gene was isolated from the lambda mouse 129-SV/J genomic DNA library (Stratagene) and used to construct the RyR2 exon-3 deletion (Ex3-del) knock-in (KI) targeting vector.

    Article Title: The glutamate receptor GluN2 subunit regulates synaptic trafficking of AMPA receptors in the neonatal mouse brain.
    Article Snippet: The N-methyl-D-aspartate receptor (NMDAR) plays various physiological and pathological roles in neural development, synaptic plasticity and neuronal cell death.. It is composed of two GluN1 and two GluN2 subunits and, in the neonatal hippocampus, most synaptic NMDARs are GluN2B-containing receptors, which are gradually replaced with GluN2A-containing receptors during development.. Here, we examined whether GluN2A could be substituted for GluN2B in neural development and functions by analysing knock-in (KI) mice in which GluN2B is replaced with GluN2A.

    Article Title: Distribution and Function of Cardiac Ryanodine Receptor Clusters in Live Ventricular Myocytes
    Article Snippet: Generation of a knock-in mouse model expressing a GFP-tagged RyR2 – A genomic DNA phage clone containing part of the mouse cardiac ryanodine receptor gene was isolated from the lambda mouse 129-SV/J genomic DNA library (Stratagene) and used to construct the RyR2 GFP knockin (KI) targeting vector.

    Mouse Assay:

    Article Title: The Transmembrane Adaptor Protein LIME Is Essential for Chemokine-Mediated Migration of Effector T Cells to Inflammatiory Sites
    Article Snippet: .. Generation of LIME Mice For the 5’ arm, an 8.0-kb SalI-EcoRI fragment upstream of the first exon was obtained from a LIME phage clone obtained by screening a lambda library made from the 129 Sv mouse strain (Stratagene). ..



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    Image Search Results


    Human multiple myeloma cell line U266-ffluc was i.v. (tail vein) injected into NSG mice. After 4 weeks, the mice were randomized into 3 groups comprising 8–9 mice each and treated with i.v. (tail vein) injections of 3 mg/kg of the ADC, the unconjugated antibody, and vehicle alone. (A) Human lambda light chain concentrations correlating with tumor burden were determined by ELISA at the indicated time points and plotted as mean ± SD values. The p value (t-test) compares the ADC group to the unconjugated antibody group. (B) Kaplan-Meier survival curve. The p value (log-rank test) compares the ADC group to the unconjugated antibody group. See also Figure S3 and Table S3.

    Journal: Cell chemical biology

    Article Title: Stable and potent selenomab-drug conjugates

    doi: 10.1016/j.chembiol.2017.02.012

    Figure Lengend Snippet: Human multiple myeloma cell line U266-ffluc was i.v. (tail vein) injected into NSG mice. After 4 weeks, the mice were randomized into 3 groups comprising 8–9 mice each and treated with i.v. (tail vein) injections of 3 mg/kg of the ADC, the unconjugated antibody, and vehicle alone. (A) Human lambda light chain concentrations correlating with tumor burden were determined by ELISA at the indicated time points and plotted as mean ± SD values. The p value (t-test) compares the ADC group to the unconjugated antibody group. (B) Kaplan-Meier survival curve. The p value (log-rank test) compares the ADC group to the unconjugated antibody group. See also Figure S3 and Table S3.

    Article Snippet: Human Lambda ELISA Quantitation Set , Bethyl Laboratories , Cat#E80-116.

    Techniques: Injection, Enzyme-linked Immunosorbent Assay

    (A) Human breast cancer cell line KPL-4 was xenografted into the mammary fat pads of female NSG mice, grown to ~150 mm3, randomized into 5 groups comprising 6 mice each, and treated with i.v. (tail vein) injections of the indicated ADCs and controls four times every week at 5 mg/kg. Mean ± SD values are plotted. (B) Four female CD-1 mice were injected i.v. with 6 mg/kg of anti-HER2 scFv-Fc(Ser396Sec)/CN29. The plasma concentrations of total antibody and intact ADC were quantified by ELISA at the indicated time points, using HER2 ECD for capturing and either anti-His mAb (total antibody) or anti-MMAF mAb (intact ADC) for detection. Shown are mean ± SD values for each time point. See also Figure S5, Figure S6, Figure S7, and Table S6.

    Journal: Cell chemical biology

    Article Title: Stable and potent selenomab-drug conjugates

    doi: 10.1016/j.chembiol.2017.02.012

    Figure Lengend Snippet: (A) Human breast cancer cell line KPL-4 was xenografted into the mammary fat pads of female NSG mice, grown to ~150 mm3, randomized into 5 groups comprising 6 mice each, and treated with i.v. (tail vein) injections of the indicated ADCs and controls four times every week at 5 mg/kg. Mean ± SD values are plotted. (B) Four female CD-1 mice were injected i.v. with 6 mg/kg of anti-HER2 scFv-Fc(Ser396Sec)/CN29. The plasma concentrations of total antibody and intact ADC were quantified by ELISA at the indicated time points, using HER2 ECD for capturing and either anti-His mAb (total antibody) or anti-MMAF mAb (intact ADC) for detection. Shown are mean ± SD values for each time point. See also Figure S5, Figure S6, Figure S7, and Table S6.

    Article Snippet: Human Lambda ELISA Quantitation Set , Bethyl Laboratories , Cat#E80-116.

    Techniques: Injection, Clinical Proteomics, Enzyme-linked Immunosorbent Assay

    KEY RESOURCES TABLE

    Journal: Cell chemical biology

    Article Title: Stable and potent selenomab-drug conjugates

    doi: 10.1016/j.chembiol.2017.02.012

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Human Lambda ELISA Quantitation Set , Bethyl Laboratories , Cat#E80-116.

    Techniques: Virus, Recombinant, Marker, Conjugation Assay, Proliferation Assay, Enzyme-linked Immunosorbent Assay, Quantitation Assay, Software